Composite

Part:BBa_K346032

Designed by: yang hu   Group: iGEM10_Peking   (2010-10-14)

T7promoter(I719005)_RBS(B0034)_MBP(mercury metal binding peptide)

This part is a combination of T7promoter(I719005),RBS(B0034) and MBP(mercury metal binding peptide). MBP is short for metal binding peptide. It is designed by our team by duplicating MerR's metal binding sites and connect them using a link.

By constructing this part, we are able to express MBP in the cytosol to bind heavy metal within the cell.

Mbpp.jpg

Figure 1: The structure of MBP we designed. The ball indicate the binding heavy metal. Noting that this MBP is constructed by duplicating two helix which is the metal-binding domain of MerR and connect them using a linker.

Mnbcx.jpg

Figure 2: The expression of MBP detected using SDS-PAGE and Western blotting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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