Part:BBa_K3458005:Design

Apis Mellifera Silk Fibroin 3 With Codon Optimization

Assembly Compatibility:

In order to facilitate further utilizing the part into the plant, Arabidopsis thaliana, as a model plant, is used as the chassis biology.
BBa_K1763000 is derived from bees without codon optimization and a length of 951bp. In our preliminary research, the CAI index of this part is low in common expression systems such as E. coli and Arabidopsis. (The UCLA team itself also reflected that its expression in E. coli is not ideal)
It can be inferred that the frequency of codon usage between bee and Arabidopsis thaliana is quite different. If the codon optimization of the sequence is carried out, a better expression effect may be obtained. We optimized the codon of BBa_K1763000 according to the codon preference of Arabidopsis thaliana, which provided a basis for the study of expressing this protein in plants.

The sequence of honeybee silk protein 3 comes from BBa_K1763000(2015 UCLA)

Weisman, S., Haritos, V., Church, J., Huson, M., Mudie, S., Rodgers, A., Dumsday, G.,

Sutherland, T. Honeybee silk: Recombinant protin production, assembly, and fiber spinning. Elsevier Ltd. 2009.

Sutherland, T., Church, J., Hu, X., Huson, M., Kaplan, D., Weisman, S. Single Honeybee Silk Protein Mimics Properties fo Multi-Protein Silk. PLoS ONE 2011. e16489

Sutherland, T. Conservation of Essential Design Features in Coiled Coil Silks. Mol Biol Evol 2007;24:2424-2432