Part:BBa_K3456000:Experience

The plasmid was successfully transformed into Escherichia coli BL21. After expression and centrifugation, there was no fluorescence visible. More tests need to be performed to see if the inhibitor protein is actually expressed.

Applications of BBa_K3456000

The part of the inhibitor can be used to purify ATP synthase. Runswick Michael J., Bason John V., Montgomery Martin G., Robinson Graham C., Fearnley Ian M. and Walker John E. 2013 The affinity purification and characterization of ATP synthase complexes from mitochondriaOpen Biol.3120160 http://doi.org/10.1098/rsob.120160

It has also the ability to block the ATP hydrolysis but not the synthesis of ATP of the ATP synthase. This can be especially useful if only the synthesis is desired.

The whole construct can be purified with its his tag through a nickel-coated column.

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