Part:BBa_K3456000:Design

I1-60GFPHis - ATPase Inhibitor with GFP and His-Tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We designed the part with a 17x helix linker between the I1-60 and the eGFP to add some space between these basic parts. It remains to be tested if the linker has any influence on the functionality.

Source

The original IF1 amino acid sequence is: MAATALAART RQAVWSVWAM QGRGFGSESG DNVRSSAGAV RDAGGAFGKR EQAEEERYFR ARAKEQLAAL KKHHENEISH HAKEIERLQK EIERHKQSIK KLKQSEDDD

It originates from Bos taurus (Bovine). UniProtKB - P01096 (ATIF1_BOVIN)

References

The procedure for the affinity purification was first mentioned in the paper "The affinity purification and characterization of ATP synthase complexes from mitochondria" (Runswick Michael J., Bason John V., Montgomery Martin G., Robinson Graham C., Fearnley Ian M. and Walker John E. 2013 The affinity purification and characterization of ATP synthase complexes from mitochondria Open Biol.3120160 http://doi.org/10.1098/rsob.120160).