Tag

Part:BBa_K3454045

Designed by: Zhiyu Fang   Group: iGEM20_ShanghaiTech_China   (2020-10-27)

MBP-tag

MBP, maltose binding protein, servers as common protein tags.

Usage and Biology

MBP fusion can significantly improve protein solubility.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55


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