Part:BBa_K3453125
Rosewood DmMatK Trigger 2.2 transcription unit
This part is a transcription unit of the Rosewood DmMatK Trigger 2.2 (BBa_K3453025) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).
Usage and Biology
A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
Figure 1. Toehold switches principle.
This part is a transcription unit of BBa_K3453025 which is a trigger for the Rosewood DmMatK Toehold Switch 2.2 (BBa_K3453015).
This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.
Full results are available on the BBa_K3453115 page in the registry.
References
[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 60
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 60
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 60
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 60
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 60
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 19
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