Part:BBa_K3453122
Rosewood DmMatK Trigger 1.3 transcription unit
This part is a transcription unit of the Rosewood DmMatK Trigger 1.3 (BBa_K3453022) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).
Usage and Biology
A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA thereby exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
Figure 1. Toehold switches principle.
This part is a transcription unit of BBa_K3453022 which is a trigger for the Rosewood DmMatK Toehold Switch 1.3 (BBa_K3453012) and also contains the switch binding site for the Rosewood DmMatK Toehold Switches 1.1 (BBa_K3453011) and 1.4 (BBa_K3453013).
This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.
Full results are available on the BBa_K3453111, BBa_K3453112 and BBa_K3453113 pages in the registry.
References
[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 102
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