DNA

Part:BBa_K3453001

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-03)


Golden Gate adapter with BsaI cloning sites

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite part BBa_K3453101 and allows rapid engineering of reporter constructs using Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None