Plasmid

Part:BBa_K3452888

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-APX2

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9193
    Illegal PstI site found at 9181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3055
    Illegal XhoI site found at 2012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9193
    Illegal PstI site found at 9181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9193
    Illegal PstI site found at 9181
    Illegal AgeI site found at 4559
    Illegal AgeI site found at 4960
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9169


[edit]
Categories
Parameters
None