Coding

Part:BBa_K3450019

Designed by: Priya Sharma   Group: iGEM20_IISER_Bhopal   (2020-10-26)

CesT: Type-3 secretion system substrate chaperone

CesT is a T3SS-specific multi-cargo chaperone found in E. coli [1] that interacts with multiple bacterial effectors. It was initially discovered as a chaperone of Tir (translocated intimin receptor). [2]

References

[1] Thomas, N. A. et al. CesT is a multi-effector chaperone and recruitment factor required for the efficient type III secretion of both LEE- and non-LEE-encoded effectors of enteropathogenic Escherichia coli. 57, 1762–1779 (2005). [2] Ye, F., Yang, F., Yu, R. et al. Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT. Nat Commun 9, 1196 (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 203
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None