Coding

Part:BBa_K3450016:Design

Designed by: Priya Sharma   Group: iGEM20_IISER_Bhopal   (2020-10-23)


Coding sequence of TEV Protease with a single S219V mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed the extra bases upstream to the start codon, stop codon, and extra bases downstream to the stop codon.

Codon optimized for Escherichia coli.

Removed the BsaI site from the sequence to make it RFC1000 compatible.

Source

TEV virus genome

References