Part:BBa_K3450007

β-intimin Neae: Intimin is an important virulence factor of EHEC and mediates intimate adhesion

The N-terminus of β-intimin, termed Neae, is a stable transmembrane base, facilitating the display of proteins fused to its C-terminus on E. coli’s surface. It is derived from the cell surface adhesin β-intimin from enterohemorrhagic E. coli (EHEC), responsible for binding to the Type III Secretion System effector Tir (Translocated Intimin Receptor), displayed on the apical surface of intestinal epithelial cells.

When used to display proteins, β-intimin exhibited the ability to serve as a non-toxigenic base that did not leave its chassis susceptible to membrane damage. It has been used to display single-domain antibodies (sdAbs) on E. coli’s surface, directing the adhesion of its chassis to in vivo tumour cells bearing target antigens.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 529
  Illegal PstI site found at 607
  Illegal PstI site found at 619
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 529
  Illegal PstI site found at 607
  Illegal PstI site found at 619
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 529
  Illegal PstI site found at 607
  Illegal PstI site found at 619
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 529
  Illegal PstI site found at 607
  Illegal PstI site found at 619
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  Illegal NgoMIV site found at 801
  Illegal NgoMIV site found at 1542
  
• 1000
  COMPATIBLE WITH RFC[1000]