Composite

Part:BBa_K3447110

Designed by: Yushan Geng, Yu Ma   Group: iGEM20_Jilin_China   (2020-10-27)


The expression of AIP

This is a part that can produce AIP constitutively.


Usage and Biology

The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. We use these two parts together to produce AIP constitutively.
Characterization To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).

Figure 1. Digestion and electrophoresis of agrBD.

Design

Design Notes

We added some synonymous mutations to avoid part rules.


Source

We found this sequence data in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None