Part:BBa_K3447009

agrA and agrC, an AIP sensing element

The sensing element of AIP.

Usage and Biology

AgrC and AgrA constitute a two-part system. AgrC is the receptor for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P₂ promoter of the agr_BDCA operon to form a positive feedback loop. The activity of AgrA dimers will also combine with P₃ promoter, to activate the non-coding RNA, the RNA III expression. RNA III can affect the virulence factor and adhesion factor of the translation process.

Figure 1. Diagram of the Agr system of Staphylococcus aureus

In our project, We realize the induction to AIP by constitutive expression of agrA and agrC. The effectiveness of the system was validated by attaching fluorescent proteins downstream of P₂ BBa_K3447011.
AIP induces green fluorescent protein expression: BBa_K3447111

Design

Design Notes

We added some synonymous mutations to avoid part rules.

Source

We found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]