Composite

Part:BBa_K3445001:Design

Designed by: Kaleb Ramon   Group: iGEM20_Alma   (2020-10-25)


Estrogen responsive RFP output


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed this composite part in the fashion it is in as a way to disallow for the bleed through of RFP from an upstream promoter. Since the Tet repressible promoter is the only promoter upstream of RFP, no bleed through transcription/translation can occur.


Source

DNA from the 2019 iGEM distribution kit (I13521) and creation by IDT labs (K123002).

References