Composite

Part:BBa_K3440021:Design

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)


MtrB under oscillatory circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2324
    Illegal NheI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4050
    Illegal BamHI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3093


Design Notes

Electricity production under oscillatory circuit in Shewanella oneidensis


Source

pLux from Vibrio fischeri MtrB from Shewanella oneidensis RhlR from Pseudomonas aeruginosa pRhl from Pseudomonas aeruginosa RhlI from Pseudomonas aeruginosa AiiA from Bacillus thuringiensis LuxR from Vibrio fischeri

References