Composite

Part:BBa_K3440020:Design

Designed by: Victoria Muliadi, Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


Oscillation circuit under GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1733
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 3249


Design Notes

GFP reporter of oscillation in E.coli


Source

RhlR from Pseudomonas aeruginosa pRhl from Pseudomonas aeruginosa RhlI from Pseudomonas aeruginosa AiiA from Bacillus thuringiensis GFP from Aequorea victoria

References