Intermediate

Part:BBa_K3440019

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)


MtrB under Lux promoter

Pconst(BBa_J23100) - RBS(BBa_B0034) - LuxR(BBa_C0062) - DT(BBa_B0015) - Plux(BBa_R0062) - RBS(BBa_B0034) - mtrb(BBa_K3146001) - DT(BBa_B0015)

Usage and Biology

This is an intermediate part of the oscillatory system which can be used to build our final modular biosensor BBa_K3440021. LuxR which is originally from Vibrio Fischeri (UniProtKB - P12746), is constitutively expressed under the J23100 promoter. In the presence of 3OC6-HSL (a lactone produced by LuxI from Vibrio Fischeri, UniProtKB - P12747), the LuxR:3OC6-HSL complex can activate Plux, which controls production of MtrB. This oscillatory part will be present in Shewanella oneidensis MR-1 mtrb knockout. As this part is an intermediate in making the final oscillatory system it cannot be characterized individually unless added into BBa_K3440021.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


[edit]
Categories
//classic/signalling/receiver
Parameters
proteinsmtrb, LuxR