Composite

Part:BBa_K3440018:Design

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)


PCB detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
    Illegal BglII site found at 2108
    Illegal BamHI site found at 1301
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320
    Illegal SapI.rc site found at 1284


Design Notes

Full construct for detection of PCB and expression of LuxI


Source

bphR2 from Pseudomonas oleovorans bphR1 promoter from Pseudomonas oleovorans LuxI from Vibrio fischeri

References