Composite

Part:BBa_K3440017:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


bphR2 under bphR1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
    Illegal BamHI site found at 1301
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320
    Illegal SapI.rc site found at 1284


Design Notes

Show that bphR1 promoter can be activated by expression of bphR2 (and better with PCB)


Source

bphR2 from Pseudomonas oleovorans

References