Composite

Part:BBa_K3440011:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


GFP under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

This part was designed to show that the constitutive promoter J231000 works. A GFP reporter was added to help monitor activity. A modeare RBS was used (BBa_B0034)

Source

GFP from Aequorea victoria (Jellyfish)

References