Composite

Part:BBa_K3440001:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


mCherry under bphR1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


Design Notes

Test bphR1 promoter leakiness and compare expression with/without PCB. The part produces mCherry to facilitate characterisation. THe part was put under a moderate strength RBS (BBa_B0034)

Source

mCherry from Anaplasma marginale

References