Composite

Part:BBa_K3431048:Design

Designed by: Huan-Jui Chang, Cheng-Ruei Yang, Hung-Yu Chen   Group: iGEM20_CSMU_Taiwan   (2020-10-17)


T7 promoter + RBS + mRFP + T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We combined the mRFP sequence from BBa_E1010 with T7 promoter, RBS (BBa_B0034), and T7 Terminator to ensure that this device can be expressed in PURExpress in vitro protein synthesis kit.


Source

This is a synthetic construct suitable to express monomeric RFP derived from DsRed in the work of Campbell, R. E. et al.

Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences, 99(12), 7877-7882.

References