Composite
Part:BBa_K3431044:Design
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-10-17)
zr146_B_ToeholdSwitch-Regulated Invertase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 40
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 40
Illegal NheI site found at 1421 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 40
Illegal BglII site found at 1192
Illegal BamHI site found at 1322
Illegal XhoI site found at 1393 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 40
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 40
Illegal AgeI site found at 993 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We measure the thermodynamic characteristic of toehold switch and measure the glucose concentration with glucose meter to determine the efficacy of our composite device.
Source
This is a synthetic construct based on the work of Green et.al.
Green, A. A., Silver, P. A., Collins, J. J., & Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell, 159(4), 925-939.