Regulatory

Part:BBa_K3431005:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-07-21)


op21_A Toehold Switch for miR-21 Detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure its minimal free energy is in an appropriate range (lower than 0 kcal/mole)


Source

This is a synthetic sequence based upon Green, A.A. et al. and Wang, S. et al. for detection of miRNA-21. We obtained this sequence through custom gene synthesis of IDT.

References

Green, A. A., Silver, P. A., Collins, J. J., & Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell, 159(4), 925-939.

Wang, S., Emery, N. J., & Liu, A. P. (2019). A novel synthetic toehold switch for microRNA detection in mammalian cells. ACS synthetic biology, 8(5), 1079-1088.