Generator

Part:BBa_K343002:Design

Designed by: Christian Kurtzhals   Group: iGEM10_SDU-Denmark   (2010-07-02)


ninaB Gene on constitutive promotor, with rbs and dual terminators.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 746
    Illegal BamHI site found at 481
    Illegal BamHI site found at 1738
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1342
    Illegal BsaI site found at 1790


Design Notes

promoter strength was chosen, to limit expression of the enzyme.


Source

coding sequence from cDNA promoter from the anderson collection rbs from the anderson collection double terminator from the registry

References