Regulatory

Part:BBa_K3425138:Design

Designed by: Pedro Nunes   Group: iGEM20_UofUppsala   (2020-10-25)


Promoter ydfJ115


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part, we had to make sure that the binding site for ydfI (mentioned in the reference of this page) was included in the sequence.

Source

Sequence obtained from the genome of B. subtilis strain w168.

References

Serizawa, M., and Sekiguchi, J. (2005) The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily. Microbiology. 151, 1769–1778