Composite
pyeaR GFP

Part:BBa_K3425131:Design

Designed by: Emelie B. & Therese K.   Group: iGEM20_UofUppsala   (2020-10-21)


PyeaR GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A standard E. coli RBS (BBa_B0034) and terminator (BBa_B0010) were used.

The GFP used was codon optimized for E. coli expression and based on the basic part BBa_E0040.