Regulatory
PyeaR

Part:BBa_K3425130:Design

Designed by: Emelie Bringeland, Therese Koivula   Group: iGEM20_UofUppsala   (2020-10-17)

YeaR Regulatory Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part, we had to make sure that the binding site for NarL was included in the sequence.

Source

The sequence was obtained from the E. coli strain DH5α.

References

Lin, H.-Y., Bledsoe, P. J., and Stewart, V. (2007) Activation of yeaR-yoaG Operon Transcription by the Nitrate-Responsive Regulator NarL Is Independent of Oxygen- Responsive Regulator Fnr in Escherichia coli K-12. JB. 189, 7539–7548