Regulatory

Part:BBa_K3425105:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-23)


DBD-CaFF construct (pSB3C11-J23108)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2713
    Illegal NheI site found at 1399
    Illegal NheI site found at 2736
    Illegal NheI site found at 2759
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2713
    Illegal BglII site found at 2832
    Illegal BglII site found at 3706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1313
    Illegal AgeI site found at 3398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 6


Design Notes

Assembled with TypeIIS.


Source

Same as BBa_K3425100.

References