Regulatory
Part:BBa_K3425101:Design
Designed by: Rubén Crespo Blanco Group: iGEM20_UofUppsala (2020-10-08)
pCadBA operator and promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constitutive expression of BBa_K3425100 has leaky expression, but dimerization increases the expression of downstream translational units notably. Native CadC may induce expression too, but we didn't found evidence of this on LB or other typically used growth media.
Source
Rhee, J. E., Kim, K. S., and Choi, S. H. (2005). CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. Journal of bacteriology, 187(22), 7870-7875.