Composite
Part:BBa_K3425053
Designed by: Tereza Hubackova Group: iGEM20_UofUppsala (2020-10-18)
Qβ3' recognition sequence of Qβ replicase with terminator
BBa_K3425053 is a construct containing the Qβ3' recognition sequence of Qβ replicase, BBa_K3425039, fused with the standard terminator BBa_B0015.
QB recognition site
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 15
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 46
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/bacteriophage
//chassis/prokaryote/ecoli
//classic/rna/uncategorized
//direction/reverse
//chassis/prokaryote/ecoli
//classic/rna/uncategorized
//direction/reverse
chassis | E. coli |
device_type | RNA functional element |
function | RNA amplification |