Plasmid_Backbone

Part:BBa_K3425004:Design

Designed by: N�ria Garriga Alonso   Group: iGEM20_UofUppsala   (2020-10-19)


pSB3C14: Improved pEven4 Loop Vector based on pSB3C04


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal NheI site found at 1399
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 6


Design Notes

A PCR mutagenesis was designed and conducted on pSB3C01 to remove the illegal BsaI restriction site. The primers used for this mutagenesis are Mut_L2_Fw and Mut_L2_Rev and they can be found in Team Uppsala 2020's Parts page.


Source

PCR mutagenesis from pSB3C01.

References