Regulatory

Part:BBa_K3424024:Design

Designed by: Julie Mallinger   Group: iGEM20_UFlorida   (2020-10-27)


RiboJ-Insulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf

References

Clifton, K., Jones, E., Paudel, S., Marken, J., Monette, C., Halleran, A., et al. (2018). The genetic insulator RiboJ increases expression of insulated genes. Journal of Biological Engineering. 12(23). doi: 10.1186/s13036-018-0115-6. </p>

Luo, C., Stanton, B., Chen, Y., Munsky, B. and Voight, C. (2012). Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nat Biotechnol. 30(11): 1137-1142.

Meyer, A., Segall-Shapiro, T., Glassey E., Zhang, J. and Voigt, C. (2019). Escherichia coli “Marionnette” strains with 12 highly optimized small-molecule sensors. Nature Chemical Biology. 15:196-204.