Terminator

Part:BBa_K3424009:Design

Designed by: David G   Group: iGEM20_UFlorida   (2020-10-23)


Terminator_L3S3P21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 46


Design Notes

Source

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE


References

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3