Regulatory

Part:BBa_K3424006:Design

Designed by: David G   Group: iGEM20_UFlorida   (2020-10-23)


PhoBR promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is part of the PhoBR operon for Escherichia coli K-12. The sequence was obtained from RegulonDB.


Source

http://regulondb.ccg.unam.mx/operon?term=ECK120014866&organism=ECK12&format=jsp&type=operon#phoBp

References

Uluşeker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramanoğulları O. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 2019 Feb 14;9(1):2076. doi: 10.1038/s41598-018-38223-w. PMID: 30765722; PMCID: PMC6376016.

A dynamic model of the phosphate response system with synthetic promoters in Escherichia coli Cansu Uluşeker , Jesus Torres , José L. García , Martin M. Hanczyc , Juan Nogales , and Ozan Kahramanoğulları Artificial Life Conference Proceedings 2017 NO. 29, 412-419