Part:BBa_K342003:Experience

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Applications of BBa_K342003

We managed a quantification of biofilms formation of a mutant OmpR234 strain (PHL818 chassis, more explained above), versus a wild-type strain (MG1655), at 30°C, in a 24-well hydrophobic polystyrene maden plate. Optimal condition to observe biofilms is a growth in minimal medium (M63/2), supplemented with a carbon source (glucose 0,2g/L). We modified osmotic pressure by varying sucrose concentration (between 0 and 0,3 mol/L). The purpose of this experiment is to make in evidence the osmolarity influence on the capacity to OmpR234 to induce Curli adherent structures.

As we can see, a decreasing adherence percentage of OmpR234 mutant is observed (about 40%), while the osmotic pressure is decreased (increasing medium osmolarity). The most important biofilm formation is seen without any osmolyte (sucrose 0 mol/L). We tested the wild-type strain for adherence without sucrose. We observed that the OmpR234 mutation induced a slight increase in biofilm formation (about 7%). This experiment is a preliminarly characterisation of our OmpR234 BioBrick, as the part was designed from this genomic mutant protein.
Thus, we expected to have a higher effect with a high-copy plasmid containing our OmpR234 BioBrick (under a constitutive promoter for example).

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