Coding

Part:BBa_K3419004:Design

Designed by: Sophia Windemuth   Group: iGEM20_Cornell   (2020-10-17)


mCardinal with strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 492
    Illegal BsaI.rc site found at 681


Design Notes

To maximize production, the strongest promoter available was used.


Source

Comes from a modified version of mNeptune, a protein produced by Entacmaea quadricolor, a sea anemone. It is combined with a strong promoter, strong ribosome binding site, and terminator.

References