Part:BBa_K341458

Chloramphenicol resistance(with Promoter) + I-sceI site+ Recombination site

Chloramphenicol resistance gene(with Promoter) + I-sceI site+ Recombination site+DraIII cutting site. This part can be used as a recombination unit between a plasmid and the chromosome in the "In-vivo Recombination System" in iGEM project "Tsinghua 2010"

This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61

This part is uesd as a Insertion Fragment of Donor Plasmid in the In-vivo Recombination System of iGEM Tsinghua 2010 Project.

Donor Plasmid contains several Insertion Fragments and each Insertion Fragment contains following five parts:

1) one 15bp-long restriction enzyme I-scel recognition site

2) one 25bp-long random sequence

3) one fragment for insertion and recombination

4) one 25bp-long random sequence

5) one 15bp-long restriction enzyme I-scel recognition site(in correspondence with 1)

Donor plasmid contains several Insertion Fragments. Based on the flanking landing pad sequence, we can ascribe Insertion Fragments to the same group as long as the flanking landing pad sequences of those Insertion Fragments are the same.

In order to achieve different goals, we design different Donor Plasmids, different Donor Plasmids contain different number of Insertion Fragments, which belong to different groups.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]