Other

Part:BBa_K341001:Design

Designed by: Sheng WANG   Group: iGEM10_Tsinghua   (2010-10-20)

attB site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 924
    Illegal XbaI site found at 934
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 924
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 924
    Illegal BamHI site found at 644
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 924
    Illegal XbaI site found at 934
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 924
    Illegal XbaI site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 938


Design Notes

11bp-601bp： Replication origin

669bp-899bp: attP

Source

from vecter puk2

References