Composite

Part:BBa_K3409010:Design

Designed by: Albane Mabro   Group: iGEM20_Ionis_Paris   (2020-10-17)


Microcin PDI cluster genes for McpM, McpI, McpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used the Salis Lab Algorithm to predict the Translation Initiation Rates of each start codon (according to different combinations of Promoters and Ribosome Binding Sites) to make sure the mRNA was translated in similar levels to the native genes from the cluster. One promoter (strength 0.58) for all three Coding Sequences and different RBS for each CDS to avoid repetition. Used a double terminator to assure the end of transcription. Synthesis provided by IDT. The presence of all 5 genes from the cluster are essential for appropriate expression and secretion of Microcin PDI.


Source

This is a composite part including the mcpM, mcpI, and mcpA coding regions encoded in BBa_K3409003, BBa_K3409004, BBa_K3409005 respectively.

References