Coding

Part:BBa_K3409000:Design

Designed by: Charlotte Duteil   Group: iGEM20_Ionis_Paris   (2020-10-19)


Gene Product (gp) 37


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 292
    Illegal BglII site found at 1486
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1666
    Illegal AgeI site found at 634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1869


Design Notes

Position 469: GAA (Glu) mutated to GAG (Glu) to remove illegal EcoRI site


Source

Entire genomic sequence of the bacteriophage T4 found on NCBI GenBanK: https://www.ncbi.nlm.nih.gov/nuccore/NC_000866.4?report=genbank&to=168903

Specific sequence for gp37 found on NCBI GenBank : https://www.ncbi.nlm.nih.gov/nuccore/NC_000866.4?from=156542&to=159622

References