Composite

Part:BBa_K3408008:Design

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-07)


Pnar-B0034-CⅠ-B0015-PCⅠ-B0034-GFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1709


Design Notes

We used this part to make sure that our CⅠ protein can function normally in Bacillus subtilis.

Source

We designed it by combining our basic parts Pnar(BBa_K3408000) and optimized CⅠ(BBa_K3408004).

References