RNA

Part:BBa_K3408002:Design

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-03)


Trigger RNA(of toehold)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added some bps to trigger RNA in the 5' terminal to increase its stability.

We have optimized the Trigger RNA on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.

Source

We found it from the paper https://pubs.acs.org/doi/10.1021/acssynbio.8b00488, and we improved it according to the paper https://aiche.onlinelibrary.wiley.com/doi/full/10.1021/bp9801143

References

[1] Carrier T A , Keasling J D. Library of Synthetic 5' Secondary Structures To Manipulate mRNA Stability in Escherichia coli[J]. Biotechnology Progress, 2010,15(1):58-64.

[2] Kim Soo-Jung,Leong Matthew,Amrofell Matthew B,Lee Young Je,Moon Tae Seok. Modulating Responses of Toehold Switches by an Inhibitory Hairpin.[J]. ACS synthetic biology,2019,8(3).