![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K3402060:Design
Designed by: Yibo Shi Group: iGEM20_Jiangnan_China (2020-10-25)
Cas9 expression cassette without homologous arms
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5675
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1730
Illegal BglII site found at 5294
Illegal XhoI site found at 1051
Illegal XhoI site found at 1780
Illegal XhoI site found at 2143
Illegal XhoI site found at 5095 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5675
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5675
Illegal NgoMIV site found at 2557
Illegal NgoMIV site found at 3661 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1501
Illegal BsaI site found at 2218
Design Notes
n
Source
Starmerella bombicola