Regulatory

Part:BBa_K3402044

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-22)


Pmob

Pmob is the promoter of monooxygenase B from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 26
    Illegal BglII site found at 684
    Illegal XhoI site found at 341
    Illegal XhoI site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 103
    Illegal SapI site found at 630
    Illegal SapI site found at 815


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Parameters
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