Regulatory

Part:BBa_K3402041

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-22)


Pfao2

Pfao2 is the promoter of putative fatty alcohol oxidase 2 gene from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Pfao2 by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 778
    Illegal PstI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 778
    Illegal PstI site found at 1090
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 778
    Illegal PstI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 778
    Illegal PstI site found at 1090
    Illegal NgoMIV site found at 77
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None