Coding

Part:BBa_K3402023:Design

Designed by: Zhihang Zhang   Group: iGEM20_Jiangnan_China   (2020-10-19)


Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 3815
    Illegal XhoI site found at 301
    Illegal XhoI site found at 664
    Illegal XhoI site found at 3616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 2182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI site found at 739


Design Notes

n


Source

n

References