Regulatory

Part:BBa_K3402005

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-01)


Peno

Peno is the promoter of enolase from Starmerella bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Peno by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 475
    Illegal XhoI site found at 1189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90


[edit]
Categories
Parameters
None