Coding

Part:BBa_K3398006

Designed by: MingXiao Wei   Group: iGEM20_ZJU-China   (2020-10-17)


scFv_Fc protein with FLAG tag

This part is encodes a fusion protein of single-chain variable fragment (scFv) and fragment crystallizable (Fc) with a FLAG tag introduced at the C-terminums for separation from other proteins.An enterokinase cleavage site for cleavage after purification was inserted followed the tag. For more detailed information about the fusion protein of scFv and ZZ, we have submitted it as BBa_K3398005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1140
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 807


[edit]
Categories
Parameters
None